Structure and Function of Organisms
Bio 206L

Lab 13: Frog sciatic nerve

Questions for Frog Nerve Lab

 

1.  The nerve used in the experiment this week is named the ___________ nerve.

2.  The nerve to used in the experiment this week innervates the frog's _________.

3.  The action potential is initiated in the nerve in response to stimulation that exceeds a critical value known as ___________.

4.  The action potential is displayed by the use of a digital _________________.

5.  The nerve contains a large number of axons; therefore the action potential measured is not from a single axon but the sum of the action potential from many axons; the action potential measured is therefore called the ______________ action potential.

7 & 8.  Conduction velocity is determined as _____________ divided by _____________.

9.  Multiple components in the action potential are seen as a consequence of different axons having diameters and different states of myelination, resulting in these axons having different ____________ ____________.

10.  The stimulus that evokes an action potential from all the axons in the nerve is known as the ______________ stimulus.

11. The amplitude of the action potential increases as the stimulus is increased beyond threshold because additional _________ become active.

12 & 13. To make the recording, the nerve is placed across a series of wires in a plastic box.  Two of these electrodes are used for ______________ and two are used for _____________.

14. The action potential that is first recorded when the nerve is first placed in the plastic box has two phases and is known as _____________.

15. To convert the action potential from one having two phases to one having only a single phase one must ________ the nerve between the two recording electrodes.

16. Two of the wires from the plastic nerve box are connected to a differential amplifier.  This amplifier records the ____________ in potential between its two inputs.

17. The delay control on the stimulator will control the time between the application of the stimulus and the beginning of the oscilloscope ______________.

18. The amplitude of the action potential is measured in units called ____________ (unit should include power of ten).

19. The duration of the action potential is measured in units called ___________ (unit should include power of ten).

20. The ____________ period is the time after an action potential during which the nerve is unable to generate a second action potential.

 

 


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