Structure and Function of Organisms
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Lab 3: Microscopy IIQuestions for Second Microscopy Lab
1. _______________ is the property of some compounds that they absorb light at some wavelength and shortly thereafter emit light at a slightly longer wavelength. 2. In the lab, the fluorescence microscopes we use are said to be epi-fluorescence microscopes, because the illumination reaches the specimen through the __________ lens. 3. ________________ immunofluorescence is the technique in which the fluorochrome is attached to the antibody directed against the molecule of interest. 4. ________________ immunofluorescence is the technique in which the fluorochrome is attached to a "secondary" antibody directed against the "primary" antibody. 5. The __________ filter is used to insure that only the light coming from the excited fluorochrome reaches the observer (or the camera). 6. The __________ filter is used to insure that the only the light appropriate for excitation of fluorochrome reaches the specimen. 7. The use of antibodies in fluorescence is the technique known as ___________________. 8. To visualize the binding of a rabbit primary antibody, one would use a fluorochrome-conjugated anti-____________. 9. To visualize the binding of a mouse monoclonal antibody, one could use a fluorochrome-conjugated anti-_____________. 10. To visualize the DNA in cells one can use a fluorescent compound known as _________ (two possible answers). 11. In the microscope to be used for fluorescence, the filters necessary to observe each individual fluorochrome are located in a small box known as a ________ _________. 12. A common red fluorochrome used in biology is ____________. 13. A common green fluorochrome used in biology is ____________. 14. The purpose of the dichroic (or mirror) in the fluorescence microscope is to _________ wavelengths of light that excite the fluorochrome. 15. The purpose of the dichroic in the fluorescence microscope is to ____________ wavelengths of light that are emitted by the fluorochrome. 16. The L8 cells to be used in lab are derived from _______ (species of animal). 17. The L8 cells to be used in lab can make what kind of tissue? ___________ 18. A paramecium is an example of what kind of organism? ____________ 19. Elodea comes from what kingdom? __________ 20. In transmission fluorescence microscopes, there is no dichroic because the light exciting the fluorochrome reaches the specimen by passing through the _______ lens. 21. The reason an object appears red in a fluorescence microscope is because the object emits _______ light. 22. An object will appear blue in the fluorescence microscope if it emits ________ light. 23. To change the fluorochrome in use in an epi-fluorescence microscope, one changes the _____ _________ (two words).
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