matzLABMETHODS
cDNA amplification from microscopic amounts on animal tissue From: Generation of cDNA libraries: Methods and protocols (Shao-Yao Ying Ed.) Humana press, pp. 103-116.Methods_files/Matz%20cDNA%20amplification%202003.pdfMethods_files/Porites%20Stress%20DB-3.htmlshapeimage_2_link_0
SMART-RACE: obtaining unknown cDNA flanks
    From: Generation of cDNA libraries: Methods and protocols (Shao-Yao Ying Ed.) Humana press, pp. 41-50.
Sequencing and de novo transcriptome analysis using 454
   cDNA preparation protocol  
   (for 454 Titanium)  
   bioinformatic scripts
   step-by-step bioinformatics guide
   BMC genomics paper
 
qRT-PCR data processing for linear mixed model analysis
  Preprint of the paper (in review at BMC Genetics):
CAmethod_ms.pdf
CAmethod_1.0.tar.gz  
   This is an R source package, containing several core CAmethod functions to process data (ResNorm function to select most stable control genes using Residuals method). Also includes functions  PrimEff to calculate amplification efficiency from dilution series, and function CAexplore to verify that the data satisfy linear model assumptions.
Download  the file to your computer and install within R as a local source package.
Simulation scripts from CAmethod manuscript:
    sim1_3methods.R (regulation effects)
    sim2_corr_coreg.R (effects of co-regulation and correlation)
2b-RAD: cost-efficient whole-genome genotyping
   library preparation protocol   (version 2, Sept 10, 2011)
 
budget RNA-seq: global gene expression profiling for $100 / sample
   library preparation   (version 3, July 19, 2011)
    pdf of the paper: Meyer et al Mol Ecol 2011