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BIO 160L - Immunology Lab |
| Course Summary |
This is an introductory, applied immunology laboratory course, which highlights the historic and current methodologies that have been important for elucidation and diagnosis of immune functions.
This course is designed to help students integrate the information they have acquired from their lecture based courses; such as immunology BIO360K, and to apply this information within the real world.
Research principles, quantitative reasoning, and understanding of immunological research methodologies will be highlighted within the course. |
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| Topics Covered |
The following topics will be covered during the course:
- Laboratory safety procedures
- Common laboratory techniques
- Important quantitative laboratory skills
- Calculating dilutions
- Graphical presentation of data
- Interpretation of quantitative data
- Experimental design and controls
- Blood morphology
- Whole white cell and differential cell counts
- Assessing T cell functions
- Dissection and tissue isolation
- Sterile technique and eukaryotic cell tissue culture
- Microscopy
- Bright field, phase contrast and fluorescent microscopy
- Protein purification and purification assessement
- Separation and detection assays
- Precipitin based assays
- ELISA
- Immunoelectrophoresis
- Western Blotting
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| NOTE: |
The course is designed to supplement and support the Immunology course BIO360K. |
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